The movie shows a z-stack (250 ?m square) taken from the pial surface to a depth of 350 ?m, every 2 ?m. The depth is indicated every 10 ?m in the bottom left corner. The left panel shows labeling with SR101, which selectively labels astrocytes only. The center panel shows the labeling with OGB1, which labels both neurons and astrocytes. The right panel shows merged images of the two channels, color-coded such that neurons appear yellow-green and astrocytes appear purple-white. Credit: James Schummers, MIT Full story